Combination of Bioaugmentation and Biostimulation in WWTP, Lamongan Fish Market As A Control Effort of Kaliotik River Pollution

Abstract

Bioaugmentation and biostimulation are In Situ bioremediation with an engineered degradation model. Each of these method models has advantages and disadvantages, so a combination of the two is needed as an alternative that can accelerate the degradation process. This study aims to determine whether biostimulation and bioaugmentation or a combination of both can accelerate the degradation of the Lamongan Fish Market liquid waste. The parameters that will be analyzed quantitatively in this study are physics and chemistry which refers to the quality standards of PermenLHK N0. P.68 of 2016. Quantitative analysis tests include turbidity, TDS, TSS, pH, DO, Nitrate, Ammonia, BOD, COD. The treatments consisted of biostimulation (BS), bioaugmentation (BG) and a mixture of both (BB), K- (without aeration), K+ (with aeration). The test sample was taken from wastewater at the inlet of the Lamongan Fish Market WWTP. This research method will be carried out using a laboratory experiment using a completely randomized design with a factorial pattern with three replications with a batch reactor processing system. The first factor is the variation of the tested treatment samples (BS, BG, BB, K-, and K+) with the second factor being the incubation time (0, 1, 2, 3 weeks). The implementation of augmentation is by adding as much as 2% (v/v) per 100 mL of water sample (bacterial suspension with Aλ: 610nm = 0.5) of EM4. Meanwhile, the addition of liquid fertilizer to biostimulation was carried out at a concentration of 0.5% (v/v) to determine its effect on the combination of bioaugmentation and biostimulation. Incubation was carried out at room temperature aerobically by applying an aerator in each batch. In the biostimulation treatment, the parameter that experienced the largest percentage decrease was ammonia, which was 97%. And in the augmentation treatment, the parameters that experienced the largest percentage decrease were ammonia by 99% and nitrate by 74%. While the combination of the two experienced the largest percentage decrease, namely in ammonia 96% and nitrate at 85%. The high percentage of ammonia reduction was due to the high protein content of organic waste in wastewater at the Lamongan Fish Market WWTP, which was 115 mg/L, which did not meet the quality standards. The best ammonia reduction was in the bioaugmentation treatment which reached 0.6 mg/L on incubation day 21 when compared to the negative control (without treatment).